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Research Objectives


L.A. Holland and L.D. Casto-Boggess, Gels in Microscale ElectrophoresisAnnual Reviews in Analytical Chemistry 2023, 16 (1), 161-179.

T. A. Shaffer, C.U. Herrada, A.M. Walker, L.D. Casto-Boggess, L.A. Holland*,  T.R. Johnson, M.E. Jones, and Y.S. Elshamy, A Cost-Effective Microfluidic Device to Teach the Principles of Electrophoresis and Electroosmosis. Journal of Chemical Education2023. 100(7)L p. 2782-2788

L.D. Casto-Boggess, L.A. Holland*, P.A. Lawer-Yolar, J.A. Lucas, and J.R. Guerrette, Microscale Quantification of the Inhibition of Neuraminidase Using Capillary Nanogel Electrophoresis. Analytical Chemistry, 2022. 94(6): pp. 16151–16159.

T.R. Johnson, T.A. Shaffer, L.A. Holland*, L.M. Veltri, J.A. Lucas, Y.S. Elshamy, and P.K. Rutto, A Low-Cost and Simple Demonstration of Freezing Point Depression and Colligative Properties with Common Salts and Ice Cream. Journal of Chemical Education, 2022. 99(10): pp. 3590-3594.

Y.S. Elshamy, T.G. Strein, L.A. Holland*, C. Li, A. DeBastiani, S.J. Valentine, P. Li, J.A. Lucas, and T.A. Shaffer, Nanoflow Sheath Voltage-Free Interfacing of Capillary Electrophoresis and Mass Spectrometry for the Detection of Small Molecules. Analytical Chemistry, 2022. 94(32): pp. 11329-11336.

L.A. Holland*, Y. He, J.R. Guerrette, C.L. Crihfield, and L. Bwanali, Simple, rapid, and reproducible capillary gel electrophoresis separation and laser-induced fluorescence detection of DNA topoisomers with unmodified fused silica separation capillaries. Analytical and Bioanalytical Chemistry, 2022. 414(1): pp. 713-720.

L. Bwanali, and L.A. Holland*, Capillary Nanogel Electrophoresis for the Determination of the β1–4 Galactosyltransferase Michaelis–Menten Constant and Real-Time Addition of Galactose Residues to N-Glycans and Glycoprotein. Analytical Chemistry, 2021. 93(34): pp. 11843-11851.

L.A. Holland*, Capillary Electrophoresis In Comprehensive Glycoscience 2nd Edition, Barchi, J., Ed.; Elsevier. volume 1, 2021, pp. 290-308.  ISBN: 9780128194751

Crihfield, C.L., and L.A. Holland*, Protein Sieving with Capillary Nanogel Electrophoresis, Analytical Chemistry, 2021, 93, pp. 1537–1543.

Veltri, L.M. and L.A. Holland*, Microfluidics for Personalized Reactions to Demonstrate Stoichiometry. Journal of Chemical Education. 2020, 97, pp. 1035-1040.

Crihfield, C.L., C.J. Kristof, L.M. Veltri, C.A. Wilson, W.M. Penny, and L.A. Holland*, Semi-permanent Cationic Coating for Protein Separations. Data in Brief, 2020. 29: pp. 105123.

Kristoff, CJ, Li, C. Li, P. L.A. Holland*, Vibrating Sharp-Edge Spray Ionization Interface for Capillary Electrophoresis and Mass Spectrometry, Analytical Chemistry. 2020. 92:  pp. 3006-3013 

Bwanali, L., C.L. Crihfield, E.O. Newton, V. Zeger, S. Gattu, and L.A. Holland*, Quantification of the α2-6 sialic acid linkage in branched N-glycan structures with capillary nanogel electrophoresis. Analytical Chemistry. 2020. 92(1): pp. 1518-1524.

Kristoff, C.J., L. Bwanali, L.M. Veltri, G.P. Gautam, P.K. Rutto, E.O. Newton, and L.A. Holland*, Challenging Bioanalyses with Capillary Electrophoresis Analytical Chemistry, 2020. 92(1): pp. 49-66.

Peng Li, Xiaojun Li, Stephen J. Valentine and Lisa Holland, US Provisional Patent Application #62/872,702, Devices and processes for mass spectrometry utilizing vibrating sharp-edge spray ionization, filed July 11, 2019.

Crihfield, C.L., C.J. Kristoff, L.M. Veltri, W.M. Penny, and L.A. Holland*, Semi-permanent cationic coating for protein separations. Journal of Chromatography A, 2019. 1607(6): pp. 46039.

Lu, G., and L.A. Holland*, Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis. Analytical Chemistry. 2019. 91(2): pp. 1375–1383.

Holland, L. * and W. Zhong, Analytical Developments in Advancing Safety in Nanotechnology. Analytical and Bioanalytical Chemistry, 410(24): 6037-6039 2018.

Holland, L.A. *, J.S. Carver, L.M. Veltri, R.J. Henderson, and K.D. Quedado, Enhancing Research for Undergraduates through a Nanotechnology Training Program That Utilizes Analytical and Bioanalytical Tools. Analytical and Bioanalytical Chemistry, 2018. 410(24): pp. 6041-6050.

Holland, L.A., US Non-Provisional Patent Application #62655859, Microfluidic Device and Uses of Teaching Scientific Principles, filed April 11, 2018

Holland, L.A. and S. Gattu, US Non-Provisional Patent Application, 15/911624 "Phospholipid Nanogel for Enzyme Stabilization". 2018, West Virginia University.

Hernandez, P. R.*, P.D. Hopkins, K. Masters, L. Holland, B.M. Mei, M. Richards-Babb, K. Quedado, N.J. Shook, E.L. Dolan, Student Integration into STEM Careers and Culture: A Longitudinal Examination of Summer Faculty Mentors and Project Ownership, CBE—Life Sciences Education, 2018. 17(3): Article 50: pp. 1-14 

Gattu, S, C.L. Crihfield, G. Lu, Bwanali, L., L.M. Veltri, L.A. Holland*, Enzyme Substrate Analysis with Capillary Electrophoresis, Methods, 2018. 146: pp. 93-106. 

Lu G., C.L. Crihfield, S. Gattu, L.M. Veltri, L.A. Holland*, Capillary electrophoresis separations of glycans, Chemical Reviews, 2018. 118: pp. 7867-7885.

Davis, T.A., S.M. Patberg, A. Stefaniak, L. Sargent, and L.A. Holland*, Affinity Capillary Electrophoresis Method to Assess Carboxylation of Multi-walled Carbon Nanotubes. Analytica Chimica Acta, 2018. 1027: pp. 149-157. 

T.A. Davis and L.A. Holland*, Peptide Probe for Multi-walled Carbon Nanotubes: Electrophoretic Assessment of the Binding Interface and Evaluation of Surface Functionalization. ACS Applied Materials and Interfaces, 2018. 10: pp. 11311-11318.

L.A. Holland*, S. Gattu, C.L. Crihfield, and L. Bwanali, Capillary Electrophoresis with Stationary Nanogel Zones of Galactosidase and Erythrina Cristagalli Lectin for the Determination of β(1-3)-Linked Galactose in Glycans. Journal of Chromatography A, 2017. 1523: pp. 90-96.

S. Gattu, C.L. Crihfield, L.A. Holland*, “Microscale Measurements of Michaelis-Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of Sialic Acid LinkageAnalytical Chemistry, 2017. 89(1): pp. 929-936.

B.A. Bachert; S.J. Choi; A.K. Snyder; R.V. Rio; B.C. Durney; L.A. Holland; K. Amemiya; S.L. Welkos; J.A. Bozue; C.K. Cote; R. Berisio; S. Lukomski*, A Unique Set of the Burkholderia Collagen-like Proteins Provides Insight into Pathogenesis, Genome Evolution and Niche Adaptation, and Infection Detection PLOS ONE, 10(9): e0137578.

V.T. Nyakubaya, B.C. Durney, M.C. Ellington, A.D. Kantes, P.A. Reed, S.E. Walter, J.R. Stueckle, L.A. Holland*, Rapid Capillary Electrophoresis Separations for the Determination of Circulating Steroids in 5 Microliters of Plasma from Individual Female Zebrafish Using Stacking to Achieve Nanomolar Detection Limits with UV-visible Absorbance Detection, Analytical Bioanalytical Chemistry, 407(23):6985-93, 2015.

B.C. Durney, C.L. Crihfield, L.A. Holland*, Capillary Electrophoresis Applied to DNA and RNA: Determining and Harnessing Sequence, Modifications, or Structure to Advance Bioanalyses (2009-2014), Analytical and Bioanalytical Chemistry, 407(23):6923-38, 2015.

B.C. Durney, B.A. Bachert, H.S. Sloane, S. Lukomski, J.P. Landers, L.A. Holland*, Reversible Phospholipid Nanogels for DNA Fragment Size Determinations up to 1,500 Base Pairs and Integrated Sample Stacking, Analytica Chemica Acta, 880(0):136-44, 2015.

T.A. Davis, S.L. Athey, M.L. Vandevender, C.L. Crihfield, C.C. Kolanko, S. Shao, M.C. Ellington, J.K. Dicks, J.S. Carver, L.A. Holland*. Electrolysis of Water in the Secondary Science Laboratory with Inexpensive Hand-Held Microfluidics, Journal of Chemical Education, 92(1): 116–119, 2015.

R.M. Williams, C.L. Crihfield, S. Gattu, L.A. Holland, L.J. Sooter*, In vitro selection of a single-stranded DNA molecular recognition element against atrazine, International Journal of Molecular Sciences 15(8):14332-47, 2014.

K. Tuntevski, B.C. Durney, A.K. Snyder, P.R. LaSala, A.P. Nayak, B.J. Green, D.H. Beezhold, R.V.M. Rio, L.A. Holland, S. Lukomski*, Aspergillus collagen-like (acl) genes: identification, sequence polymorphism and assessment for PCR-based pathogen detection, Applied and Environmental Microbiology, 79(24), 7882-95, 2013.

S. Boulanos, T.A. Davis, J.A. Yang, S.E. Lohse, A. Alkilany, L.A. Holland, C.J. Murphy*, Nanoparticle-protein interaction: thermodynamic and kinetic study of adsorption of bovine serum albumin to gold nanoparticle surface, Langmuir, 29 (48), 14984-14996, 2013.

B. Ramakrishnan, A.M. Moncrief, T.A.Davis, L.A. Holland, P.K. Qasba*, Investigations on b1,4-Galactosyltransferase I using 6-sulfo-GlcNAc as acceptor sugar substrateGlycoconjugate Journal , 30(9), 835-42, 2013.

B.C. Durney, J.A. Lounsbury, B.L. Poe, J.P. Landers, L.A. Holland*, Thermally Responsive Phospholipid Pseudo-gel: Tunable DNA Sieving with Capillary Electrophoresis Analytical Chemistry, 85(14), 6617-6625, 2013.

X. Wu, T.J. Langan, B.C. Durney, L.A. Holland*, A Thermally Responsive Phospholipid Preparation for Fluid Steering and Separation in Microfluidics, Electrophoresis, 33, 2674–81, 2012.

T.J. Langan, L.A. Holland*, Capillary electrophoresis coupled to electrospray mass spectrometry through a coaxial sheath flow interface and semi-permanent phospholipid coating for the determination of oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid, Electrophoresis 33, 607-613, 2012.

T.J. Langan, V.T. Nyakubaya, L.D. Casto, T.D. Dolan S.A. Archer-Hartmann, S.L. Yedlapalli, L.J. Sooter, L.A. Holland*, Assessment of Aptamer-Steroid Binding Using Stacking Enhanced Capillary Electrophoresis, Electrophoresis 33, 866-869, 2012.

S.A. Archer-Hartmann, C.L. Crihfield, L.A. Holland*, On-Line Enzymatic Sequencing of Glycans from Trastuzumab by Phospholipid Assisted Capillary Electrophoresis, Electrophoresis 32, 39491-3498, 2011.

S.A. Archer-Hartmann, L.A. Holland*, Self-Assembled Nanomaterials for Enhanced Chemical Separations, LC-GC North America, 29(5), pp. 384-393, 2011.

S.A. Archer-Hartmann, L.M. Sargent, D. Lowry, L.A. Holland*, Microscale Exoglycosidase Processing and Lectin Capture of Glycans with Phospholipid Assisted Capillary Electrophoresis Separations, Analytical Chemistry, 83(7), pp. 2740-2047, 2011.

L.A. Holland*, Capillary Electrophoresis: Focus on Undergraduate LaboratoryJournal of Chemical Education, 88(3), pp 254–256, 2011.

C.M. White, L.A. Holland*, P. Famouri, Application of Capillary Electrophoresis to Predict Crossover Frequency of Polystyrene Particles in Dielectrophoresis, Electrophoresis, 31(15), pp. 2664-2671, 2010

L. Bykova, SA. Archer-Hartmann, L.A. Holland*, L Iwanowicz, VS Blazer, Steroid Determination in Fish Plasma Using Capillary Electrophoresis, Environmental Toxicology and Chemistry, 29(9), pp. 1950–1955, 2010.

R. Luo, S.A. Archer-Hartmann, L.A. Holland*, Transformable Capillary Electrophoresis for Oligosaccharides Separation Using Phospholipid Additives, Analytical Chemistry , 82, pp. 1228-1233 2010.