Skip to main content
Holland Group
Research Objectives
Eberly College of Arts and Science
WVU Home

Publications

L.N. Taylor, L.A. Holland,* M.T. Witzel, “Native Capillary Nanogel Electrophoresis Assay of Inhibitors of Neuraminidases Derived from H1N1 and H5N1 Influenza A Pandemics”, Analytical Chemistry, 2025, 97(9) Pages 5077-5084.

C. Li., B.L. Mendis, LA. Holland, and P. Li,* Investigation of the impact of liquid presence on the acoustic streaming generated by a vibrating sharp tip capillary. Microfluidics and Nanofluidics, 2024;  28: 17.

L.C. Casto, and L.A. Holland*, Parallel Capillary Nanogel Electrophoresis Assay for the Quantification of Enzyme Inhibition. Analytica Chimica Acta 2024 Vol. 1296 Pages 342268

Witzel, M.T., L.M. Veltri, M. Kostelic, J.A. Lucas, Y.S. Elshamy, S. Lai, C. Du, V.H. Wysocki, and L.A. Holland*, Protein Analysis Using Capillary Electrophoresis Coupled to Mass Spectrometry Through Vibrating Sharp-Edge Spray Ionization. Electrophoresis 2024, 45(17-18), 1597-1605.

L.A. Holland and L.D. Casto-Boggess, Gels in Microscale Electrophoresis, Annual Reviews in Analytical Chemistry 2023, 16 (1), 161-179.

T. A. Shaffer, C.U. Herrada, A.M. Walker, L.D. Casto-Boggess, L.A. Holland*,  T.R. Johnson, M.E. Jones, and Y.S. Elshamy, A Cost-Effective Microfluidic Device to Teach the Principles of Electrophoresis and Electroosmosis. Journal of Chemical Education. 2023. 100(7)L p. 2782-2788

L.D. Casto-Boggess, L.A. Holland*, P.A. Lawer-Yolar, J.A. Lucas, and J.R. Guerrette, Microscale Quantification of the Inhibition of Neuraminidase Using Capillary Nanogel Electrophoresis. Analytical Chemistry, 2022. 94(6): pp. 16151–16159.

T.R. Johnson, T.A. Shaffer, L.A. Holland*, L.M. Veltri, J.A. Lucas, Y.S. Elshamy, and P.K. Rutto, A Low-Cost and Simple Demonstration of Freezing Point Depression and Colligative Properties with Common Salts and Ice Cream. Journal of Chemical Education, 2022. 99(10): pp. 3590-3594.

Y.S. Elshamy, T.G. Strein, L.A. Holland*, C. Li, A. DeBastiani, S.J. Valentine, P. Li, J.A. Lucas, and T.A. Shaffer, Nanoflow Sheath Voltage-Free Interfacing of Capillary Electrophoresis and Mass Spectrometry for the Detection of Small Molecules. Analytical Chemistry, 2022. 94(32): pp. 11329-11336.

L.A. Holland*, Y. He, J.R. Guerrette, C.L. Crihfield, and L. Bwanali, Simple, rapid, and reproducible capillary gel electrophoresis separation and laser-induced fluorescence detection of DNA topoisomers with unmodified fused silica separation capillaries. Analytical and Bioanalytical Chemistry, 2022. 414(1): pp. 713-720.

L. Bwanali, and L.A. Holland*, Capillary Nanogel Electrophoresis for the Determination of the β1–4 Galactosyltransferase Michaelis–Menten Constant and Real-Time Addition of Galactose Residues to N-Glycans and Glycoprotein. Analytical Chemistry, 2021. 93(34): pp. 11843-11851.

L.A. Holland*, Capillary Electrophoresis In Comprehensive Glycoscience 2nd Edition, Barchi, J., Ed.; Elsevier. volume 1, 2021, pp. 290-308.  ISBN: 9780128194751

Crihfield, C.L., and L.A. Holland*, Protein Sieving with Capillary Nanogel Electrophoresis, Analytical Chemistry, 2021, 93, pp. 1537–1543.

Veltri, L.M. and L.A. Holland*, Microfluidics for Personalized Reactions to Demonstrate Stoichiometry. Journal of Chemical Education. 2020, 97, pp. 1035-1040.

Crihfield, C.L., C.J. Kristof, L.M. Veltri, C.A. Wilson, W.M. Penny, and L.A. Holland*, Semi-permanent Cationic Coating for Protein Separations. Data in Brief, 2020. 29: pp. 105123.

Kristoff, CJ, Li, C. Li, P. L.A. Holland*, Vibrating Sharp-Edge Spray Ionization Interface for Capillary Electrophoresis and Mass Spectrometry, Analytical Chemistry. 2020. 92:  pp. 3006-3013 

Bwanali, L., C.L. Crihfield, E.O. Newton, V. Zeger, S. Gattu, and L.A. Holland*, Quantification of the α2-6 sialic acid linkage in branched N-glycan structures with capillary nanogel electrophoresis. Analytical Chemistry. 2020. 92(1): pp. 1518-1524.

Kristoff, C.J., L. Bwanali, L.M. Veltri, G.P. Gautam, P.K. Rutto, E.O. Newton, and L.A. Holland*, Challenging Bioanalyses with Capillary Electrophoresis Analytical Chemistry, 2020. 92(1): pp. 49-66.

Peng Li, Xiaojun Li, Stephen J. Valentine and Lisa Holland, US Provisional Patent Application #62/872,702, Devices and processes for mass spectrometry utilizing vibrating sharp-edge spray ionization, filed July 11, 2019.

Crihfield, C.L., C.J. Kristoff, L.M. Veltri, W.M. Penny, and L.A. Holland*, Semi-permanent cationic coating for protein separations. Journal of Chromatography A, 2019. 1607(6): pp. 46039.

Lu, G., and L.A. Holland*, Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis. Analytical Chemistry. 2019. 91(2): pp. 1375–1383.

Holland, L. * and W. Zhong, Analytical Developments in Advancing Safety in Nanotechnology. Analytical and Bioanalytical Chemistry, 410(24): 6037-6039 2018.

Holland, L.A. *, J.S. Carver, L.M. Veltri, R.J. Henderson, and K.D. Quedado, Enhancing Research for Undergraduates through a Nanotechnology Training Program That Utilizes Analytical and Bioanalytical Tools. Analytical and Bioanalytical Chemistry, 2018. 410(24): pp. 6041-6050.

Holland, L.A., US Non-Provisional Patent Application #62655859, Microfluidic Device and Uses of Teaching Scientific Principles, filed April 11, 2018

Holland, L.A. and S. Gattu, US Non-Provisional Patent Application, 15/911624 "Phospholipid Nanogel for Enzyme Stabilization". 2018, West Virginia University.

Hernandez, P. R.*, P.D. Hopkins, K. Masters, L. Holland, B.M. Mei, M. Richards-Babb, K. Quedado, N.J. Shook, E.L. Dolan, Student Integration into STEM Careers and Culture: A Longitudinal Examination of Summer Faculty Mentors and Project Ownership, CBE—Life Sciences Education, 2018. 17(3): Article 50: pp. 1-14 

Gattu, S, C.L. Crihfield, G. Lu, Bwanali, L., L.M. Veltri, L.A. Holland*, Enzyme Substrate Analysis with Capillary Electrophoresis, Methods, 2018. 146: pp. 93-106. 

Lu G., C.L. Crihfield, S. Gattu, L.M. Veltri, L.A. Holland*, Capillary electrophoresis separations of glycans, Chemical Reviews, 2018. 118: pp. 7867-7885.

Davis, T.A., S.M. Patberg, A. Stefaniak, L. Sargent, and L.A. Holland*, Affinity Capillary Electrophoresis Method to Assess Carboxylation of Multi-walled Carbon Nanotubes. Analytica Chimica Acta, 2018. 1027: pp. 149-157. 

T.A. Davis and L.A. Holland*, Peptide Probe for Multi-walled Carbon Nanotubes: Electrophoretic Assessment of the Binding Interface and Evaluation of Surface Functionalization. ACS Applied Materials and Interfaces, 2018. 10: pp. 11311-11318.

L.A. Holland*, S. Gattu, C.L. Crihfield, and L. Bwanali, Capillary Electrophoresis with Stationary Nanogel Zones of Galactosidase and Erythrina Cristagalli Lectin for the Determination of β(1-3)-Linked Galactose in Glycans. Journal of Chromatography A, 2017. 1523: pp. 90-96.

S. Gattu, C.L. Crihfield, L.A. Holland*, “Microscale Measurements of Michaelis-Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of Sialic Acid Linkage” Analytical Chemistry, 2017. 89(1): pp. 929-936.

B.A. Bachert; S.J. Choi; A.K. Snyder; R.V. Rio; B.C. Durney; L.A. Holland; K. Amemiya; S.L. Welkos; J.A. Bozue; C.K. Cote; R. Berisio; S. Lukomski*, A Unique Set of the Burkholderia Collagen-like Proteins Provides Insight into Pathogenesis, Genome Evolution and Niche Adaptation, and Infection Detection PLOS ONE, 10(9): e0137578.

V.T. Nyakubaya, B.C. Durney, M.C. Ellington, A.D. Kantes, P.A. Reed, S.E. Walter, J.R. Stueckle, L.A. Holland*, Rapid Capillary Electrophoresis Separations for the Determination of Circulating Steroids in 5 Microliters of Plasma from Individual Female Zebrafish Using Stacking to Achieve Nanomolar Detection Limits with UV-visible Absorbance Detection, Analytical Bioanalytical Chemistry, 407(23):6985-93, 2015.

B.C. Durney, C.L. Crihfield, L.A. Holland*, Capillary Electrophoresis Applied to DNA and RNA: Determining and Harnessing Sequence, Modifications, or Structure to Advance Bioanalyses (2009-2014), Analytical and Bioanalytical Chemistry, 407(23):6923-38, 2015.

B.C. Durney, B.A. Bachert, H.S. Sloane, S. Lukomski, J.P. Landers, L.A. Holland*, Reversible Phospholipid Nanogels for DNA Fragment Size Determinations up to 1,500 Base Pairs and Integrated Sample Stacking, Analytica Chemica Acta, 880(0):136-44, 2015.

T.A. Davis, S.L. Athey, M.L. Vandevender, C.L. Crihfield, C.C. Kolanko, S. Shao, M.C. Ellington, J.K. Dicks, J.S. Carver, L.A. Holland*. Electrolysis of Water in the Secondary Science Laboratory with Inexpensive Hand-Held Microfluidics, Journal of Chemical Education, 92(1): 116–119, 2015.

R.M. Williams, C.L. Crihfield, S. Gattu, L.A. Holland, L.J. Sooter*, In vitro selection of a single-stranded DNA molecular recognition element against atrazine, International Journal of Molecular Sciences 15(8):14332-47, 2014.

K. Tuntevski, B.C. Durney, A.K. Snyder, P.R. LaSala, A.P. Nayak, B.J. Green, D.H. Beezhold, R.V.M. Rio, L.A. Holland, S. Lukomski*, Aspergillus collagen-like (acl) genes: identification, sequence polymorphism and assessment for PCR-based pathogen detection, Applied and Environmental Microbiology, 79(24), 7882-95, 2013.

S. Boulanos, T.A. Davis, J.A. Yang, S.E. Lohse, A. Alkilany, L.A. Holland, C.J. Murphy*, Nanoparticle-protein interaction: thermodynamic and kinetic study of adsorption of bovine serum albumin to gold nanoparticle surface, Langmuir, 29 (48), 14984-14996, 2013.

B. Ramakrishnan, A.M. Moncrief, T.A.Davis, L.A. Holland, P.K. Qasba*, Investigations on b1,4-Galactosyltransferase I using 6-sulfo-GlcNAc as acceptor sugar substrate, Glycoconjugate Journal , 30(9), 835-42, 2013.

B.C. Durney, J.A. Lounsbury, B.L. Poe, J.P. Landers, L.A. Holland*, Thermally Responsive Phospholipid Pseudo-gel: Tunable DNA Sieving with Capillary Electrophoresis Analytical Chemistry, 85(14), 6617-6625, 2013.

X. Wu, T.J. Langan, B.C. Durney, L.A. Holland*, A Thermally Responsive Phospholipid Preparation for Fluid Steering and Separation in Microfluidics, Electrophoresis, 33, 2674–81, 2012.

T.J. Langan, L.A. Holland*, Capillary electrophoresis coupled to electrospray mass spectrometry through a coaxial sheath flow interface and semi-permanent phospholipid coating for the determination of oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid, Electrophoresis 33, 607-613, 2012.

T.J. Langan, V.T. Nyakubaya, L.D. Casto, T.D. Dolan S.A. Archer-Hartmann, S.L. Yedlapalli, L.J. Sooter, L.A. Holland*, Assessment of Aptamer-Steroid Binding Using Stacking Enhanced Capillary Electrophoresis, Electrophoresis 33, 866-869, 2012.

S.A. Archer-Hartmann, C.L. Crihfield, L.A. Holland*, On-Line Enzymatic Sequencing of Glycans from Trastuzumab by Phospholipid Assisted Capillary Electrophoresis, Electrophoresis 32, 39491-3498, 2011.

S.A. Archer-Hartmann, L.A. Holland*, Self-Assembled Nanomaterials for Enhanced Chemical Separations, LC-GC North America, 29(5), pp. 384-393, 2011.

S.A. Archer-Hartmann, L.M. Sargent, D. Lowry, L.A. Holland*, Microscale Exoglycosidase Processing and Lectin Capture of Glycans with Phospholipid Assisted Capillary Electrophoresis Separations, Analytical Chemistry, 83(7), pp. 2740-2047, 2011.

L.A. Holland*, Capillary Electrophoresis: Focus on Undergraduate Laboratory, Journal of Chemical Education, 88(3), pp 254–256, 2011.

C.M. White, L.A. Holland*, P. Famouri, Application of Capillary Electrophoresis to Predict Crossover Frequency of Polystyrene Particles in Dielectrophoresis, Electrophoresis, 31(15), pp. 2664-2671, 2010

L. Bykova, SA. Archer-Hartmann, L.A. Holland*, L Iwanowicz, VS Blazer, Steroid Determination in Fish Plasma Using Capillary Electrophoresis, Environmental Toxicology and Chemistry, 29(9), pp. 1950–1955, 2010.

R. Luo, S.A. Archer-Hartmann, L.A. Holland*, Transformable Capillary Electrophoresis for Oligosaccharides Separation Using Phospholipid Additives, Analytical Chemistry , 82, pp. 1228-1233 2010.